#6171 SEROPREVALENCE OF COVID-19 INFECTION BASED ON IGG ANTIBODY AGAINST NUCLEOCAPSID PROTEIN (NP) AND SPIKE PROTEIN (S) IN HEMODIALYSIS PATIENTS

Abstract Background and Aims Maintenance hemodialysis (MHD) patients have been identified as a high-risk group for COVID-19 infection. Serological testing can be used to monitor disease prevalence & evaluate screening measures protocols aiming at limiting transmission within dialysis units. This study was conducted observe the sero of infection among maintenance patients. Method The during 3rd wave covid-19 Total 194 in-center MHD were included from three centers. Relevant history regarding symptom, testing, managements vaccination collected. During our serum sample collected test IgG antibody against nucleocapsid protein (NP) (cut-off positive when >1.5) spike (S) >50 AIU/L) tested by Chemiluminescent microparticle immunoassay (CMIA) method developed Abbott (FDA-EUA approved). Results Mean age subjects 48±12 years where male 60%. Duration 31±22 (6-120) months. Renal pathology DN in 31%, possible GN 31%,HTN 19% rest others .From records past covid clinically diagnosed 39% which 10% based on RT-PCR another 29% fell into suspected category (i.e. symptom with/or suggestive serology). Presentation pattern mild 75%, moderate 15% (requiring oxygen), severe 5% hospitalization) asymptomatic 5%. Vaccine availed 38%. In vaccinated vs. non-vaccinated NP 63% 561%(P = 0.65) S 99% 98%(P 0.56). These indicate naturally acquired immunity most before any vaccination. titer between groups 7743±8920 5386± 6839 AIU/L (p 0.06). correlation showed that has no with duration, age, BMI, Hb%, albumin or Kt/V. Similarly shows mostly significant laboratory parameters. Conclusion data demonstrate seroprevalence population only half infected SARS-CoV-2 indicates larger number are even vaccinated. Therefore studies both should identify greater until more sensitive tools established.